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Dr. Ashay H. Nandeshwar
M. D., (Ayu)(Mumbai)
(Podar Govt. Medical College)
P.G.D.M.L.T., L.Lb. (Sch)
Lecturer V.P.A.M.C.
Sangli
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Procedure 1: Preparing the blood smears
 
  • Distilled water was used to clean all the slides. The slides were then kept open to dry
  • 5 ml syringes were used to take out blood from the patients. In adult patients, the blood was taken out from a vein in the arm; and in young children the blood was taken from finger tips.
  • About 0.5 ml of blood was taken out from each patient.
  • Each blood sample was then added into a test-tube (cleaned and dried).
  • Separate droppers were used to transfer 1 drop of blood from each sample from the test-tubes to the slides.
  • The slides were then covered with Leishman stain, and were then allowed to dry for about 30 minutes each.
  • Procedure 2: Viewing the smears under a microscope and counting the cells
    1. The prepared slides were then viewed under an optical microscope.
    2. A digital camera was used to capture the images from the eye-piece.
    3. The digital images were then zoomed on a computer and then counted.
    At the start of treatment:

    Two months of treatment:

    4 months of treatment:

    6 months of treatment:

    8 months of treatment: